Neckers L, Whitesell L
Clinical Pharmacology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Am J Physiol. 1993 Jul;265(1 Pt 1):L1-12. doi: 10.1152/ajplung.1993.265.1.L1.
Antisense RNA and DNA techniques have been developed as a relatively recent approach to the specific modulation of gene expression in vitro and in vivo. This review discusses general considerations for the application of antisense techniques. We shall examine the relative advantages and disadvantages of DNA versus RNA techniques, as well as the common pitfalls peculiar to each strategy.
反义RNA和DNA技术是作为一种相对较新的方法发展起来的，用于在体外和体内特异性调节基因表达。这篇综述讨论了反义技术应用的一般注意事项。我们将研究DNA技术与RNA技术的相对优缺点，以及每种策略特有的常见陷阱。
