Pirck M, Páy A, Heberle-Bors E, Hirt H
Institute of Microbiology and Genetics, Vienna Biocenter, Austria.
Mol Gen Genet. 1993 Jul;240(1):126-31. doi: 10.1007/BF00276891.
Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.
磷蛋白磷酸酶是真核生物细胞周期的核心调控成分。我们报道了一种苜蓿2A型磷蛋白磷酸酶（pp2aMs）cDNA的克隆和测序。预测的蛋白质序列与来自甘蓝型油菜、兔子和果蝇的PP2A具有高度相似性。未发现细胞周期中pp2aMs mRNA丰度有变化。在分批细胞培养生长过程中，mRNA水平逐渐下降。在植物中，所有器官都含有pp2a转录本，但在茎中检测到最高的mRNA水平。由于Southern分析表明苜蓿中存在一个小的pp2a基因家族，不同亚型可能在各种组织和发育情况下具有特定作用，有待进一步鉴定。
