仅在发热温度下破坏脊髓灰质炎病毒的单克隆抗体。
Monoclonal antibodies that disrupt poliovirus only at fever temperatures.
作者信息
Delaet I, Boeyé A
机构信息
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
出版信息
J Virol. 1993 Sep;67(9):5299-302. doi: 10.1128/JVI.67.9.5299-5302.1993.
Abstract
Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted to empty capsids (H antigenic, 100S), and neutralization was irreversible. The rate of antibody-dependent conversion of N to H antigen increased steeply between 37 and 39 degrees C.
摘要
在36种单克隆抗体中，有3种被发现可在39℃而非37℃时导致1型脊髓灰质炎病毒不可逆失活。在37℃时的中和作用依赖于聚集，且可通过酸诱导的解聚作用逆转；在39℃时，病毒粒子（N抗原性，160S）被破坏成空衣壳（H抗原性，100S），中和作用不可逆。在37℃至39℃之间，抗体依赖性N抗原向H抗原的转化速率急剧增加。
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