Scinicariello F, Eberle R, Hilliard J K
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
J Infect Dis. 1993 Sep;168(3):747-50. doi: 10.1093/infdis/168.3.747.
Rapid diagnosis of B virus (herpesvirus simiae) infection in humans followed by early antiviral treatment is essential for the patient's survival. To improve laboratory diagnosis of B virus infections, a polymerase chain reaction (PCR)-based test using synthetic oligonucleotide primers and probe was developed to detect B virus DNA in clinical samples. After the specificity of the PCR was assessed for detection of several B virus isolates, the method was used to investigate human and monkey specimens, and results were compared with those obtained by viral culture. PCR appeared to be more sensitive than conventional virus isolation and thus of practical use for a rapid identification of B virus infection when conventional viral cultures are negative.
对人类B病毒（猴疱疹病毒）感染进行快速诊断并尽早进行抗病毒治疗对患者的存活至关重要。为了改进B病毒感染的实验室诊断方法，开发了一种基于聚合酶链反应（PCR）的检测方法，使用合成寡核苷酸引物和探针来检测临床样本中的B病毒DNA。在评估了PCR检测几种B病毒分离株的特异性后，该方法被用于研究人类和猴子的标本，并将结果与病毒培养获得的结果进行比较。PCR似乎比传统的病毒分离更敏感，因此当传统病毒培养为阴性时，对于快速鉴定B病毒感染具有实际应用价值。
