Smith A L, Black D H, Eberle R
Department of Infectious Diseases and Physiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078-2006, USA.
J Virol. 1998 Nov;72(11):9224-32. doi: 10.1128/JVI.72.11.9224-9232.1998.
Although monkey B virus (herpesvirus simiae; BV) is common in all macaque species, fatal human infections appear to be associated with exposure to rhesus macaques (Macaca mulatta), suggesting that BV isolates from rhesus monkeys may be more lethal to nonmacaques than are BV strains indigenous to other macaque species. To determine if significant differences that would support this supposition exist among BV isolates, we compared multiple BV strains isolated from rhesus, cynomolgus, pigtail, and Japanese macaques. Antigenic analyses indicated that while the isolates were very closely related to one another, there are some antigenic determinants that are specific to BV isolates from different macaque species. Restriction enzyme digest patterns of viral DNA revealed marked similarities between rhesus and Japanese macaque isolates, while pigtail and cynomolgus macaque isolates had distinctive cleavage patterns. To further compare genetic diversity among BV isolates, DNA sequences from two regions of the viral genome containing genes that are conserved (UL27 and US6) and variable (US4 and US5) among primate alphaherpesviruses, as well as from two noncoding intergenic regions, were determined. From these sequence data and a phylogenetic analysis of them it was evident that while all isolates were closely related strains of BV, there were three distinct genotypes. The three BV genotypes were directly related to the macaque species of origin and were composed of (i) isolates from rhesus and Japanese macaques, (ii) cynomolgus monkey isolates, and (iii) isolates from pigtail macaques. This study demonstrates the existence of different BV genotypes which are related to the macaque host species and thus provides a molecular basis for the possible existence of BV isolates which vary in their levels of pathogenicity for nonmacaque species.
尽管猴B病毒（猕猴疱疹病毒；BV）在所有猕猴种类中都很常见，但致命的人类感染似乎与接触恒河猴（猕猴）有关，这表明来自恒河猴的BV分离株对非猕猴的致死性可能比其他猕猴种类的本土BV毒株更高。为了确定BV分离株之间是否存在支持这一假设的显著差异，我们比较了从恒河猴、食蟹猴、猪尾猴和日本猕猴中分离出的多个BV毒株。抗原分析表明，虽然这些分离株彼此密切相关，但存在一些特定于不同猕猴种类的BV分离株的抗原决定簇。病毒DNA的限制性内切酶消化模式显示，恒河猴和日本猕猴分离株之间有显著相似性，而猪尾猴和食蟹猴分离株有独特的切割模式。为了进一步比较BV分离株之间的遗传多样性，我们测定了病毒基因组两个区域的DNA序列，这两个区域包含在灵长类α疱疹病毒中保守的基因（UL27和US6）和可变的基因（US4和US5），以及两个非编码基因间隔区。从这些序列数据及其系统发育分析可以明显看出，虽然所有分离株都是密切相关的BV毒株，但存在三种不同的基因型。这三种BV基因型与起源的猕猴种类直接相关，分别由（i）来自恒河猴和日本猕猴的分离株、（ii）食蟹猴分离株和（iii）猪尾猴分离株组成。这项研究证明了存在与猕猴宿主种类相关的不同BV基因型，从而为可能存在对非猕猴种类致病性水平不同的BV分离株提供了分子基础。
