Singh J, Wagner E K
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Virology. 1993 Sep;196(1):220-31. doi: 10.1006/viro.1993.1470.
In this study we have used a combination of Northern blot, cDNA, and RNAse protection analysis to characterize transcription occurring at the junction of the long terminal repeat (TRL) and long unique segment (UL) of herpes simplex virus type 1 (HSV-1). Two low abundance 5' colinear transcripts mapped between the junction of the TRL/UL and the cap site of the primary latency associated transcripts (LAT). Analysis of the region containing the first four open translational reading frames (ORFs) of the UL segment of the virus revealed a complex pattern of overlapping transcripts and polyadenylation site utilization. Inefficient termination late, but not early in infection, at the polyadenylation site following the UL2 ORF leads to an unusual situation where the promoter controlling a beta gamma transcript encoding the UL1 ORF also controls a 5' colinear gamma transcript. Further, although the entire UL3 ORF is conserved between HSV-1 and HSV-2, the only abundant uniquely UL3 encoding transcript in both virus types was found to originate within the UL3 ORF and has the capacity to encode only a truncated UL3 protein product.
在本研究中，我们使用了Northern印迹、cDNA和RNA酶保护分析相结合的方法，来表征单纯疱疹病毒1型（HSV-1）长末端重复序列（TRL）与长单一序列（UL）交界处发生的转录情况。在TRL/UL交界处与主要潜伏相关转录本（LAT）的帽位点之间定位到了两种低丰度的5'共线性转录本。对病毒UL区段前四个开放阅读框（ORF）所在区域的分析显示，转录本存在复杂的重叠模式以及多聚腺苷酸化位点的利用情况。在感染后期而非早期，UL2 ORF之后的多聚腺苷酸化位点处发生低效终止，导致一种异常情况，即控制编码UL1 ORF的βγ转录本的启动子也控制一个5'共线性γ转录本。此外，尽管HSV-1和HSV-2之间整个UL3 ORF是保守的，但在两种病毒类型中，唯一丰富的仅编码UL3的转录本被发现起源于UL3 ORF内，并且仅能编码一种截短的UL3蛋白产物。
