Grubman M J, Lewis S A, Morgan D O
USDA, Plum Island Animal Disease Center, Greenport, NY 11944.
Vaccine. 1993;11(8):825-9. doi: 10.1016/0264-410x(93)90357-4.
Three groups of swine were each inoculated with a different antigen preparation of foot-and-mouth disease virus (FMDV) capsid proteins and challenged by contact exposure to animals infected with FMDV. One group of four animals was inoculated with an extract from cells infected with a recombinant baculovirus containing the FMDV P1-2A structural protein precursor gene and a portion of the P2 gene. Two out of four animals were protected from clinical disease, but not from virus replication. A second group of animals was inoculated with an extract from Escherichia coli that expressed FMDV proteins from a construct containing the P1-2A gene, a portion of the P2 gene and the 3C protease gene. Three out of four animals in this group did not develop clinical signs of FMD upon challenge and two of four were protected against virus replication. In contrast, inoculation of a third group of swine with an extract from E. coli expressing the same FMDV construct as present in the recombinant baculovirus failed to protect any of the four animals from generalized FMD.
将三组猪分别接种口蹄疫病毒（FMDV）衣壳蛋白的不同抗原制剂，并通过与感染FMDV的动物接触暴露进行攻毒。一组四只动物接种了来自感染重组杆状病毒的细胞提取物，该重组杆状病毒含有FMDV P1-2A结构蛋白前体基因和部分P2基因。四只动物中有两只免受临床疾病影响，但未免受病毒复制。第二组动物接种了来自大肠杆菌的提取物，该大肠杆菌从含有P1-2A基因、部分P2基因和3C蛋白酶基因的构建体中表达FMDV蛋白。该组四只动物中有三只在攻毒后未出现口蹄疫的临床症状，四只中有两只免受病毒复制。相比之下，第三组猪接种与重组杆状病毒中相同FMDV构建体的大肠杆菌提取物，四只动物中无一免受全身性口蹄疫感染。
