Harvey R J, Kim H C, Darlison M G
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, Germany.
FEBS Lett. 1993 Oct 4;331(3):211-6. doi: 10.1016/0014-5793(93)80339-v.
We have isolated a cDNA, from the chicken, that encodes a fourth type of gamma subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name gamma 4) displays 67%, 69% and 70% identity, respectively, to the rat gamma 1, gamma 2 and gamma 3 subunits. In the developing chicken brain, the gamma 4-subunit mRNA is first detected at embryonic day 13; the transcript level then increases progressively during embryogenesis. In situ hybridization reveals that the gamma 4-subunit mRNA is abundant in several brain regions, including the ectostriatum, nucleus rotundus and hyperstriatum ventrale, which are involved in visual processing and learning.
我们从鸡中分离出了一个cDNA，它编码脊椎动物脑GABAA受体的第四种γ亚基。成熟多肽（我们命名为γ4）与大鼠γ1、γ2和γ3亚基的同一性分别为67%、69%和70%。在发育中的鸡脑中，γ4亚基mRNA在胚胎第13天首次被检测到；转录水平随后在胚胎发育过程中逐渐增加。原位杂交显示，γ4亚基mRNA在几个脑区中丰富，包括外纹状体、圆核和腹侧超纹状体，这些脑区参与视觉处理和学习。
