Henry Y, Lepoivre M, Drapier J C, Ducrocq C, Boucher J L, Guissani A
Institut Curie, INSERM U 350, Orsay, France.
FASEB J. 1993 Sep;7(12):1124-34. doi: 10.1096/fasebj.7.12.8397130.
Nitric oxide is synthesized in mammalian cells from L-arginine or from pharmaceutical drugs. It forms paramagnetic complexes with some metalloproteins, inhibiting key enzymes in DNA synthesis, mitochondrial respiration, iron metabolism, etc. This article reviews how electron paramagnetic resonance spectroscopy helps to detect unambiguously such specific molecular targets for NO in mammalian whole cells and organelles. EPR has also been used for the detection of spin adducts of free NO by spin-trapping methods.
一氧化氮在哺乳动物细胞中由L-精氨酸或药物合成。它与一些金属蛋白形成顺磁性复合物，抑制DNA合成、线粒体呼吸、铁代谢等过程中的关键酶。本文综述了电子顺磁共振波谱如何有助于明确检测哺乳动物全细胞和细胞器中一氧化氮的此类特定分子靶点。电子顺磁共振也已被用于通过自旋捕获方法检测游离一氧化氮的自旋加合物。
