Hino K, Okuda M, Konishi T, Ishiko H, Okita K
Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.
Dig Dis Sci. 1995 Jan;40(1):14-20. doi: 10.1007/BF02063935.
To determine whether the loss of serum hepatitis C virus RNA (HCV-RNA) early in interferon therapy would indicate a sustained response to this agent, we detected serum HCV-RNA successively during and after therapy. Serum samples for detection of HCV-RNA were obtained serially from 36 patients with chronic hepatitis C treated with interferon-alpha. In 28 of these patients, results of the assay were compared with genotypes and quantitative levels of HCV-RNA in serum before therapy. HCV-RNA was detected by a reverse transcription polymerase chain reaction using the 5'-noncoding region as a primer. Genotypes were determined by using type-specific primers, and serum levels of HCV-RNA were determined by a competitive reverse transcription polymerase chain reaction (RT-PCR). HCV-RNA disappeared from serum in eight of 10 responders (80%), but in only one of the 26 nonresponders (3.8%) at the second week of therapy (P < 0.0005). The time until the disappearance of HCV-RNA was correlated with the serum level of HCV-RNA present before therapy (P < 0.05). The early disappearance of HCV-RNA from serum during interferon therapy was useful in predicting a sustained response in patients with chronic hepatitis C.
为了确定在干扰素治疗早期血清丙型肝炎病毒RNA（HCV-RNA）的消失是否表明对该药物有持续反应，我们在治疗期间及之后连续检测血清HCV-RNA。从36例接受α干扰素治疗的慢性丙型肝炎患者中连续采集用于检测HCV-RNA的血清样本。其中28例患者，将检测结果与治疗前血清中HCV-RNA的基因型和定量水平进行比较。使用5'-非编码区作为引物通过逆转录聚合酶链反应检测HCV-RNA。通过使用型特异性引物确定基因型，通过竞争性逆转录聚合酶链反应（RT-PCR）确定血清中HCV-RNA的水平。在治疗的第二周，10例有反应者中的8例（80%）血清中HCV-RNA消失，但26例无反应者中仅1例（3.8%）血清中HCV-RNA消失（P<0.0005）。HCV-RNA消失前的时间与治疗前血清中HCV-RNA的水平相关（P<0.05）。干扰素治疗期间血清中HCV-RNA的早期消失有助于预测慢性丙型肝炎患者的持续反应。
