Muirhead E E, Rightsel W A, Leach B E, Byers L W, Pitcock J A, Brooks B
Lab Invest. 1977 Feb;36(2):162-72.
Transplants and lipid extracts of the same monolayer tissue culture of renomedullary interstitial cells from murine renal medulla exerted a similar antihypertensive action in rats having hypertension of the sodium-volume-dependent-type. The antihypertensive action resembled that caused by lipid extracts of rabbit renal medulla and extracts of lapine renomedullary interstitial cells grown in tissue culture. The recession of the arterial pressure of the hypertensive animals usually occurred slowly and steadily to a maximum within 6 to 12 hours. On occasions, a substantial acute depressor effect preceded the slow and steady decline of the pressure. As the pressure was lowered, there was either minimal or no change in the pulse rate. The lowering of the hypertensive pressure before there was vascularization of the transplant appears to support the view that the transplanted cells secreted and/or liberated an antihypertensive substance(s) that seeped out and was absorbed by nearby capillaries and/or lymphatics and circulated and acted in the manner of a hormone. The extracted and purified lipid from the same cells as used for transplantation is proposed as a candidate for such hormonal action. Evidence is presented that minimizes the possibility of the classic renomedullary prostaglandins as this antihypertensive lipid. The findings add support to the concept that the kidney exerts a hormonal antihypertensive action that opposes the well known hormonal prohypertensive renal actions.
来自小鼠肾髓质的肾髓质间质细胞单层组织培养物的移植体和脂质提取物，对钠容量依赖性高血压大鼠具有相似的降压作用。这种降压作用类似于兔肾髓质脂质提取物以及组织培养中生长的兔肾髓质间质细胞提取物所引起的降压作用。高血压动物的动脉血压下降通常缓慢且稳定，在6至12小时内达到最大值。有时，在血压缓慢且稳定下降之前会出现明显的急性降压效应。随着血压降低，脉搏率要么变化极小，要么没有变化。在移植体血管化之前高血压血压就降低，这似乎支持了这样一种观点，即移植的细胞分泌和/或释放了一种降压物质，该物质渗出并被附近的毛细血管和/或淋巴管吸收，然后循环并以激素的方式发挥作用。从用于移植的相同细胞中提取和纯化的脂质被认为是这种激素作用的候选物质。有证据表明经典的肾髓质前列腺素作为这种降压脂质的可能性极小。这些发现进一步支持了这样一种概念，即肾脏发挥一种激素性降压作用，与众所周知的激素性升压肾脏作用相反。
