聚合酶链反应与病毒分离及血凝试验在粪便标本中检测犬细小病毒的比较

Comparison of polymerase chain reaction with virus isolation and haemagglutination assays for the detection of canine parvoviruses in faecal specimens.

作者信息

Mochizuki M, San Gabriel M C, Nakatani H, Yoshida M, Harasawa R

机构信息

Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto, Japan.

出版信息

Res Vet Sci. 1993 Jul;55(1):60-3. doi: 10.1016/0034-5288(93)90035-e.

DOI:10.1016/0034-5288(93)90035-e

PMID:8397433

Abstract

A polymerase chain reaction (PCR) assay, which specifically amplifies the capsid gene of canine parvovirus (CPV), was compared as a diagnostic method for detecting CPV in faeces, with virus isolation (VI) on Crandell feline kidney (CRFK) or Madin-Darby canine kidney (MDCK) cells, and a faecal haemagglutination (HA) assay confirmed by inhibition with a CPV-specific antiserum. Although a false-negative result was obtained in one of 59 faecal samples (1.7 per cent) tested by the PCR assay, it was as sensitive as the VI assay using MDCK cells, and more sensitive than the VI assay using CRFK cells or the HA assay. These results indicate that the PCR assay may be useful as a routine diagnostic method for detecting CPV in faecal specimens.

摘要

一种聚合酶链反应（PCR）检测方法，该方法能特异性扩增犬细小病毒（CPV）的衣壳基因，作为检测粪便中CPV的诊断方法，与在克兰德尔猫肾（CRFK）或麦迪逊-达比犬肾（MDCK）细胞上进行病毒分离（VI）以及用CPV特异性抗血清抑制来确认的粪便血凝（HA）检测方法进行了比较。尽管在59份粪便样本中有1份（1.7%）通过PCR检测获得了假阴性结果，但它与使用MDCK细胞的VI检测方法一样灵敏，并且比使用CRFK细胞的VI检测方法或HA检测方法更灵敏。这些结果表明，PCR检测方法可能作为检测粪便标本中CPV的常规诊断方法有用。