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用于区分工艺特性优良和不良的硬粒小麦品种的低分子量谷蛋白基因单粒PCR技术

Single-seed PCR of LMW glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties.

作者信息

D'Ovidio R

机构信息

Dipartimento di Agrobiologia e Agrochimica, Università degli Studi della Tuscia, Viterbo, Italy.

出版信息

Plant Mol Biol. 1993 Sep;22(6):1173-6. doi: 10.1007/BF00028988.

DOI:10.1007/BF00028988
PMID:8400133
Abstract

Polymerase chain reaction (PCR) was used to amplify low-molecular-weight (LMW) glutenin sequences from genomic DNA extracted from a single germinating seed of several durum wheat genotypes. Electrophoretic analysis of PCR reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. This PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with good qualitative characteristics.

摘要

聚合酶链反应（PCR）用于从几种硬粒小麦基因型的单个发芽种子中提取的基因组DNA中扩增低分子量（LMW）谷蛋白序列。对PCR反应的电泳分析表明，存在具有良好和不良工艺特性的硬粒小麦品种特有的扩增产物。这种基于PCR的方法被认为是一种非常有效和安全的替代标准程序，用于选择具有良好品质特性的硬粒小麦基因型。

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引用本文的文献

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本文引用的文献

1
Chromosomal location of genes for gliadin polypeptides in durum wheat Triticum turgidum L.小麦醇溶蛋白基因在硬粒小麦中的染色体定位
Theor Appl Genet. 1983 Mar;64(4):289-93. doi: 10.1007/BF00274164.
2
Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction.利用聚合酶链反应从单粒种子中快速鉴定小麦族基因型
Plant Mol Biol. 1993 Jan;21(1):181-3. doi: 10.1007/BF00039629.
3
Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes.
开发一种新的标记系统，用于鉴定面包小麦（Triticum aestivum L.）中低分子量谷蛋白亚基基因家族的复杂成员。
Theor Appl Genet. 2011 May;122(8):1503-16. doi: 10.1007/s00122-011-1550-7. Epub 2011 Feb 23.
4
Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L).面包小麦（Triticum aestivum. L）Glu-A3等位基因低分子量谷蛋白基因的表征及标记开发
Theor Appl Genet. 2004 May;108(7):1409-19. doi: 10.1007/s00122-003-1558-8. Epub 2004 Jan 16.
5
Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer.对来自面包小麦的一种低分子量麦谷蛋白亚基基因以及代表麦谷蛋白聚合物主要亚基的相应蛋白质的表征。
Plant Physiol. 1998 Dec;118(4):1147-58. doi: 10.1104/pp.118.4.1147.
一个活性小麦低分子量麦谷蛋白基因的分子特征及其与其他小麦和大麦醇溶蛋白基因的关系。
Mol Gen Genet. 1989 Mar;216(1):81-90. doi: 10.1007/BF00332234.
4
Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction.通过聚合酶链反应扩增快速高效地检测小麦中的遗传多态性。
Plant Mol Biol. 1990 Jul;15(1):169-71. doi: 10.1007/BF00017737.
5
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.一种用于制备用于PCR分析的植物基因组DNA的简单快速方法。
Nucleic Acids Res. 1991 Mar 25;19(6):1349. doi: 10.1093/nar/19.6.1349.