通过固相微测序法进行法医DNA分型。
Forensic DNA typing by the solid-phase minisequencing method.
作者信息
Syvänen A C, Sajantila A, Lukka M
机构信息
Department of Human Molecular Genetics, National Public Health Institute, Helsinki, Finland.
出版信息
EXS. 1993;67:275-82. doi: 10.1007/978-3-0348-8583-6_25.
We describe a method for DNA-typing, in which a panel of biallelic markers are detected by the solid-phase minisequencing method (Syvänen et al., 1990). This method identifies single nucleotide variations in DNA fragments amplified by the PCR. Determination of the panel of 12 markers selected in this study proved to be an efficient and reliable method for forensic identification of individuals. We also introduce a novel approach for rapid determination of allele frequencies by quantitative analysis of pooled DNA samples.
我们描述了一种DNA分型方法，其中通过固相微测序法（Syvänen等人，1990年）检测一组双等位基因标记。该方法可识别通过PCR扩增的DNA片段中的单核苷酸变异。在本研究中选择的12个标记物面板的测定被证明是一种用于个体法医鉴定的高效且可靠的方法。我们还引入了一种通过对混合DNA样本进行定量分析来快速确定等位基因频率的新方法。
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