来自大脑cDNA文库的320个基因的染色体分布。
Chromosomal distribution of 320 genes from a brain cDNA library.
作者信息
Polymeropoulos M H, Xiao H, Sikela J M, Adams M, Venter J C, Merril C R
机构信息
Laboratory of Biochemical Genetics, National Institute of Mental Health, Neuroscience Center at St. Elizabeths, Washington, DC 20032.
出版信息
Nat Genet. 1993 Aug;4(4):381-6. doi: 10.1038/ng0893-381.
We have determined the chromosomal assignment of 320 brain expressed genes by studying the segregation of polymerase chain reaction (PCR) products in human rodent somatic cell hybrids and by genetically mapping polymorphic cDNAs using the CEPH (Centre d'Etude du Polymophisme Humaine) reference pedigrees and database. These mapped genes can function as markers on the physical map of the human genome, as well as serve as candidate disease gene loci. Distribution of these genes to the human chromosomes correlates well with the GC content of the chromosomes. However, the distribution of these genes does not correlate well with the cytogenetic length of each chromosome.
我们通过研究聚合酶链反应（PCR）产物在人-啮齿动物体细胞杂种中的分离情况，并利用CEPH（人类多态性研究中心）参考家系和数据库对多态性cDNA进行基因定位，确定了320个脑表达基因的染色体定位。这些定位的基因可作为人类基因组物理图谱上的标记，也可作为候选疾病基因位点。这些基因在人类染色体上的分布与染色体的GC含量密切相关。然而，这些基因的分布与每条染色体的细胞遗传学长度相关性不佳。
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