Hechemy K E, McKee M, Marko M, Samsonoff W A, Roman M, Baca O
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Infect Immun. 1993 Oct;61(10):4485-8. doi: 10.1128/iai.61.10.4485-4488.1993.
Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction revealed that, in heavily infected cells, the rickettsiae, in fact, reside in one multilobed vacuole. As a result of asymmetric cell division, the multilobed vacuole containing the rickettsiae apparently segregates into one daughter cell, while the companion daughter cell emerges parasite free. This likely explains the appearance of naive uninfected cells in long-term-infected (i.e., ca. 2 years) cell populations that had not been supplemented with uninfected L929 host cells.
先前通过传统透射电子显微镜对严重感染Q热普里西拉分离株的L929细胞薄片进行检查表明，立克次氏体存在于多个液泡中。本研究使用高压电子显微镜和三维重建技术发现，在严重感染的细胞中，立克次氏体实际上存在于一个多叶液泡中。由于细胞不对称分裂，含有立克次氏体的多叶液泡明显分离到一个子细胞中，而与之配对的子细胞则未被寄生虫感染。这可能解释了在未补充未感染L929宿主细胞的长期感染（即约2年）细胞群体中出现未感染幼稚细胞的现象。
