Burton P R, Stueckemann J, Welsh R M, Paretsky D
Infect Immun. 1978 Aug;21(2):556-66. doi: 10.1128/iai.21.2.556-566.1978.
Mouse fibroblasts (L-929) and Vero (green monkey kidney) cells were infected with the rickettsia Coxiella burnetti, and persistent infections developed and were studied over a 6- to 10-month period. Ultrastructural comparisons were made between the two infected cell types, and both were tested cytochemically for the presence of acid phosphatase, a marker enzyme of lysozymes. Rickettsiae were always observed within vacuoles, and some infected L cells showed flattened endoplasmic reticulum as compared with uninfected cells. Rickettsiae in Vero cells were most often seen in vacuoles containing whorls of membranes ("myelin configurations") which were also seen in uninfected cells. Rickettsiae in Vero cells were pleomorphic, with acid phosphatase reaction product in their periplasmic space. This suggests either rickettsial degradation by lysosomal enzymes which penetrated the cell envelope or a penetration after the rickettsiae were dead. Vacuoles of infected Vero cells showed much more reaction product than that in infected L cells, and most rickettsiae in L cells had a normal appearance and showed no reaction product in their periplasmic space.
将小鼠成纤维细胞（L-929）和非洲绿猴肾细胞（Vero）用伯氏考克斯体立克次氏体感染，在6至10个月的时间内形成持续性感染并进行研究。对两种受感染细胞类型进行了超微结构比较，并对两者进行了细胞化学检测，以确定溶菌酶的标记酶酸性磷酸酶的存在情况。立克次氏体总是在液泡内观察到，与未感染的细胞相比，一些受感染的L细胞显示出内质网扁平。Vero细胞中的立克次氏体最常出现在含有膜性涡旋（“髓鞘样结构”）的液泡中，未感染的细胞中也可见到这种结构。Vero细胞中的立克次氏体形态多样，其周质空间有酸性磷酸酶反应产物。这表明要么是溶酶体酶穿透细胞壁对立克次氏体进行降解，要么是立克次氏体死亡后发生了穿透。受感染的Vero细胞的液泡显示出比受感染的L细胞更多的反应产物，并且L细胞中的大多数立克次氏体外观正常，其周质空间未显示反应产物。
