Falbo V, Pace T, Picci L, Pizzi E, Caprioli A
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
Infect Immun. 1993 Nov;61(11):4909-14. doi: 10.1128/iai.61.11.4909-4914.1993.
Cytotoxic necrotizing factors (CNFs) are dermonecrotic protein toxins produced by human and animal clinical isolates of Escherichia coli. In this study, the CNF1 determinant was isolated and sequenced, showing that expression of biologically active toxin is governed by a unique open reading frame encoding a protein of 1,014 amino acids with a predicted molecular mass of 113.7 kDa. Nucleotide and protein data base searches showed significant homology between CNF1 and the dermonecrotic toxin of Pasteurella multocida. In particular, the two toxins were found to share a hydrophobic region of about 220 amino acids which is a potential membrane-spanning domain.
细胞毒性坏死因子（CNFs）是由人和动物临床分离的大肠杆菌产生的皮肤坏死蛋白毒素。在本研究中，分离并测序了CNF1决定簇，结果表明具有生物活性的毒素的表达受一个独特的开放阅读框控制，该阅读框编码一种含有1014个氨基酸的蛋白质，预测分子量为113.7 kDa。核苷酸和蛋白质数据库搜索显示CNF1与多杀巴斯德菌的皮肤坏死毒素之间存在显著同源性。特别是，发现这两种毒素共享一个约220个氨基酸的疏水区域，该区域是一个潜在的跨膜结构域。
