用于检测抗人嗜T淋巴细胞病毒I型抗体的化学发光免疫分析。
Chemiluminescence immunoassay to detect anti-HTLV-I antibodies.
作者信息
Kamihira S, Nakashima S, Saitoh S, Kawamoto M, Kawashima Y, Shimamoto M
机构信息
Blood Transfusion Service, School of Medicine, Nagasaki University.
出版信息
Jpn J Cancer Res. 1993 Aug;84(8):834-7. doi: 10.1111/j.1349-7006.1993.tb02053.x.
Abstract
A fully automated chemiluminescence immunoassay was developed for the detection of antibodies to HTLV-I. We used partially purified viral antigens coated on small polystyrene beads together with acridinium ester-labeled anti-human immunoglobulin G mouse immunoglobulin G in this method. A good agreement was observed between our proposed method, the indirect immunofluorescence assay, the particle-agglutination test and the enzyme immunoassay. This new method, which is simple, sensitive, specific and rapid, should be useful for mass screening of anti-HTLV-I antibodies.
摘要
开发了一种用于检测抗人嗜T淋巴细胞病毒I型（HTLV-I）抗体的全自动化学发光免疫分析方法。在该方法中，我们使用包被于小聚苯乙烯珠上的部分纯化病毒抗原，以及吖啶酯标记的抗人免疫球蛋白G小鼠免疫球蛋白G。我们提出的方法与间接免疫荧光法、颗粒凝集试验和酶免疫分析之间观察到良好的一致性。这种新方法简单、灵敏、特异且快速，应有助于抗HTLV-I抗体的大规模筛查。
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