人类逆转录病毒——成人T细胞白血病病毒（ATLV）的健康携带者：通过对来自外周血的携带ATLV的T细胞进行克隆培养来证明
Healthy carriers of a human retrovirus, adult T-cell leukemia virus (ATLV): demonstration by clonal culture of ATLV-carrying T cells from peripheral blood.
作者信息
Gotoh Y I, Sugamura K, Hinuma Y
出版信息
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4780-2. doi: 10.1073/pnas.79.15.4780.
Abstract
Adult T-cell leukemia virus (ATLV) or ATLV-associated antigen (ATLA)-positive cell clones were isolated from peripheral blood lymphocytes of all five anti-ATLA-seropositive healthy adults tested by a limiting-dilution culture method in the presence of T-cell growth factor (TCGF) but from none of six seronegative adults similarly tested. Although ATLA-positive cells were not always detected in mass cultures of the seropositive lymphocytes, they were found consistently in T-cell cloned cultures of those lymphocytes. Continuous cultures of the ATLA-positive clones obtained were dependent on TCGF. Five clones derived from each of the five donors were maintained for greater than 4 months and, during culture, all of them acquired the ability to grow without added TCGF. The ATLA-positive clones formed rosettes with erythrocytes and expressed Leu 1, Leu 3a, and Leu 4 antigens but not Leu 2a antigen. These results indicate that anti-ATLA-seropositive healthy individuals carry ATLV in T cells circulating in their peripheral blood.
摘要
通过有限稀释培养法，在T细胞生长因子（TCGF）存在的情况下，从所有5名接受检测的抗ATLA血清阳性健康成年人的外周血淋巴细胞中分离出成人T细胞白血病病毒（ATLV）或ATLV相关抗原（ATLA）阳性细胞克隆，但在同样检测的6名血清阴性成年人中均未分离出。虽然在血清阳性淋巴细胞的大量培养中并非总能检测到ATLA阳性细胞，但在这些淋巴细胞的T细胞克隆培养中始终能发现它们。所获得的ATLA阳性克隆的连续培养依赖于TCGF。从5名供体中各自获得的5个克隆维持培养超过4个月，并且在培养期间，所有克隆都获得了在不添加TCGF的情况下生长的能力。ATLA阳性克隆与红细胞形成玫瑰花结，并表达Leu 1、Leu 3a和Leu 4抗原，但不表达Leu 2a抗原。这些结果表明，抗ATLA血清阳性的健康个体在外周血中循环的T细胞中携带ATLV。
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Healthy carriers of a human retrovirus, adult T-cell leukemia virus (ATLV): demonstration by clonal culture of ATLV-carrying T cells from peripheral blood.人类逆转录病毒——成人T细胞白血病病毒（ATLV）的健康携带者：通过对来自外周血的携带ATLV的T细胞进行克隆培养来证明
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