Wong L L, Adler P N
Biology Department, University of Virginia, Charlottesville 22901.
J Cell Biol. 1993 Oct;123(1):209-21. doi: 10.1083/jcb.123.1.209.
The Drosophila wing is decorated with a regular array of distally pointing hairs. In the pupal wing, the hairs are formed from micro-villus like prehairs that contain large bundles of actin filaments. The distal orientation of the actin bundles reveals the proximal-distal polarity within the pupal wing epithelium. We have used F-actin staining to examine early stages of prehair development in both wild-type and mutant pupal wings. We have found a striking correlation between hair polarity and the subcellular location for assembly of the prehair. In a wild-type wing, all of the distally pointing hairs are derived from prehairs that are formed at the distal vertex of the hexagonally shaped pupal wing cells. Mutations in six tissue polarity genes result in abnormal hair polarity on the adult wing, and all also alter the subcellular location for prehair initiation. Based on their cellular phenotypes, we can place these six genes into three phenotypic groups. Double mutant analysis indicates that these phenotypic groups correspond to epistasis groups. This suggests that the tissue polarity genes function in or on a pathway that controls hair polarity by regulating the subcellular location for prehair formation.
果蝇翅膀上装饰着一系列规则排列、指向远端的刚毛。在蛹期翅膀中，这些刚毛由微绒毛状的前刚毛形成，前刚毛包含大量肌动蛋白丝束。肌动蛋白束的远端取向揭示了蛹期翅膀上皮细胞内的近端 - 远端极性。我们利用F - 肌动蛋白染色来检查野生型和突变型蛹期翅膀上前刚毛发育的早期阶段。我们发现刚毛极性与前刚毛组装的亚细胞位置之间存在显著相关性。在野生型翅膀中，所有指向远端的刚毛都源自于在六边形蛹期翅膀细胞的远端顶点形成的前刚毛。六个组织极性基因的突变会导致成虫翅膀上刚毛极性异常，并且所有这些突变还会改变前刚毛起始的亚细胞位置。基于它们的细胞表型，我们可以将这六个基因分为三个表型组。双突变分析表明这些表型组对应于上位性组。这表明组织极性基因在一个通过调节前刚毛形成的亚细胞位置来控制刚毛极性的途径中发挥作用或位于该途径上。
