Bale J F, O'Neil M E, Fowler S S, Murph J R
Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242.
J Clin Microbiol. 1993 Sep;31(9):2433-8. doi: 10.1128/jcm.31.9.2433-2438.1993.
We used the polymerase chain reaction and primers corresponding to three regions of the human cytomegalovirus (HCMV) genome to study HCMVs isolated from 16 children attending a single day-care center and the father of two children in the same center. When we analyzed isolates with primers for the pp65 and major immediate-early genes, we observed nearly uniform amplification yielding products of predicted sizes. By contrast, primers for the a sequence demonstrated variability among HCMV strains, supporting the use of these primers as an epidemiologic tool. Analysis of a-sequence products from two isolates demonstrated 50 to 70% nucleotide homology with the a sequence of HCMV Towne strain DNA. We observed 95% nucleotide homology for the two a-sequence products derived from the father-child pair. Analysis of day-care center isolates indicated that two children excreted two distinct HCMV strains during the study interval.
我们使用聚合酶链反应及与人类巨细胞病毒（HCMV）基因组三个区域对应的引物，来研究从一家日托中心的16名儿童以及同一中心两名儿童的父亲身上分离出的HCMV。当我们用针对pp65和主要立即早期基因的引物分析分离株时，观察到几乎一致的扩增，产生了预测大小的产物。相比之下，针对a序列的引物显示HCMV菌株之间存在变异性，这支持将这些引物用作流行病学工具。对两个分离株的a序列产物分析表明，其与HCMV汤氏株DNA的a序列有50%至70%的核苷酸同源性。我们观察到来自父子对的两个a序列产物有95%的核苷酸同源性。对日托中心分离株的分析表明，在研究期间，有两名儿童排出了两种不同的HCMV菌株。
