酶联免疫分析法用于艰难梭菌血清分型
Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping.
作者信息
Delmée M, Depitre C, Corthier G, Ahoyo A, Avesani V
机构信息
Microbiology Unit, University of Louvain, Brussels, Belgium.
出版信息
J Clin Microbiol. 1993 Sep;31(9):2526-8. doi: 10.1128/jcm.31.9.2526-2528.1993.
Abstract
An enzyme-linked immunoassay (ELISA) with 11 Clostridium difficile serogroup-specific antisera was applied for serogrouping of C. difficile colonies from 314 consecutive positive fecal samples. Two hundred forty-nine strains (79%) were correctly serogrouped, 57 (18%) belonged to serogroups other than the 11 which were evaluated and gave a negative reaction with all antisera, and 8 isolates (2.5%) did not react with their corresponding antisera. ELISA is a rapid and reliable method for serogrouping C. difficile and should allow for the automation of this procedure.
摘要
采用含有11种艰难梭菌血清群特异性抗血清的酶联免疫吸附测定（ELISA）对314份连续阳性粪便样本中的艰难梭菌菌落进行血清群分类。249株菌株（79%）被正确血清群分类，57株（18%）属于所评估的11种血清群以外的血清群，并且与所有抗血清均呈阴性反应，8株分离株（2.5%）与其相应抗血清不发生反应。ELISA是一种对艰难梭菌进行血清群分类的快速且可靠的方法，并且应能使该程序实现自动化。
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