Vydelingum S, Baylis S A, Bristow C, Smith G L, Dixon L K
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
J Gen Virol. 1993 Oct;74 ( Pt 10):2125-30. doi: 10.1099/0022-1317-74-10-2125.
The right variable region of the genome of a pathogenic strain of African swine fever virus (ASFV), Malawi LIL20/1, has been sequenced and 15 open reading frames (ORFs) identified by computer analysis. Eight of these ORFs were found to be similar to previously described ASFV ORFs and three of these belong to two previously described multiple gene families (MGF), 360 and 110. Four of the remaining five ORFs belong to a novel MGF, designated MGF 100, and the last ORF encodes a protein that is similar to the virus structural protein, p22. Copies of MGF 110 and the gene coding for p22 have previously been characterized only at the left end of the ASFV genome. The organization of these genes suggests evolution by duplications, deletions and sequence transposition from one end of the genome to the other. Sequence comparisons of members of MGF 360 suggest that the Malawi LIL20/1 genome has undergone separate DNA rearrangements compared to the Ba71V genome. Lastly, one ORF was found to be similar to the myeloid differentiation primary response protein, MyD116 and to the herpes simplex virus neurovirulence-associated factor ICP34.5.
对非洲猪瘟病毒（ASFV）致病菌株马拉维LIL20/1基因组的右可变区进行了测序，并通过计算机分析鉴定出15个开放阅读框（ORF）。发现其中8个ORF与先前描述的ASFV ORF相似，其中3个属于先前描述的两个多基因家族（MGF），即360和110。其余5个ORF中的4个属于一个新的MGF，命名为MGF 100，最后一个ORF编码一种与病毒结构蛋白p22相似的蛋白质。MGF 110的拷贝和编码p22的基因以前仅在ASFV基因组的左端进行过表征。这些基因的组织表明，它们是通过从基因组一端到另一端的重复、缺失和序列转座而进化的。MGF 360成员的序列比较表明，与Ba71V基因组相比，马拉维LIL20/1基因组经历了单独的DNA重排。最后，发现一个ORF与髓样分化初级反应蛋白MyD116以及单纯疱疹病毒神经毒力相关因子ICP34.5相似。
