Cuppini R, Cecchini T, Ciaroni S, Ambrogini P, Del Grande P
Institute of Morphological Sciences, University of Urbino, Italy.
J Neurol Sci. 1993 Jul;117(1-2):61-7. doi: 10.1016/0022-510x(93)90155-r.
The increased number of poly-innervated cells in normal and reinnervated extensor digitorum longus (edl) muscle of vitamin E-deficient rats suggests enhanced sprouting by motor neurons in conditions of decreased protection against lipid peroxidation. End-plates and terminal axons were observed by a combined technique that shows both end-plate acetylcholinesterase area and axons. Quantitative observations of nodal and terminal sprouting in normally innervated and reinnervated edl muscles of vitamin E-deficient rats were carried out. Branch points of nerve terminal within end-plates were also observed. Three main results were obtained. First, a notable increase of both terminal and nodal sprouting was found in reinnervated muscles of normal and vitamin E-deficient rats; moreover, a relative increase in the number of nodal sprouts occurs in the long run. Second, in muscles of uninjured, vitamin E-deficient rats, nodal and terminal sprouting and branching within end-plate was greater than in controls. Third, nodal sprouting by regenerating axons was more affected by vitamin E-deficiency than terminal sprouting and branching within end-plates.
在维生素E缺乏大鼠的正常和重新神经支配的趾长伸肌（EDL）中，多神经支配细胞数量增加，这表明在对脂质过氧化的保护作用降低的情况下，运动神经元的芽生增强。通过一种能同时显示终板乙酰胆碱酯酶区域和轴突的联合技术观察终板和终末轴突。对维生素E缺乏大鼠正常神经支配和重新神经支配的EDL肌肉中的结和终末芽生进行了定量观察。还观察了终板内神经末梢的分支点。获得了三个主要结果。第一，在正常和维生素E缺乏大鼠的重新神经支配肌肉中，终末和结芽生均显著增加；此外，从长远来看，结芽的数量相对增加。第二，在未受伤的维生素E缺乏大鼠的肌肉中，结和终末芽生以及终板内的分支比对照组更多。第三，与终板内的终末芽生和分支相比，维生素E缺乏对再生轴突的结芽生影响更大。
