Henderson G I, Schenker S
Res Commun Chem Pathol Pharmacol. 1977 Jan;16(1):15-32.
The clinical observations that offspring of pregnant alcoholics are prone to congenital defects and abnormal growth and development (fetal alcohol syndrome) have generated interest in studying this problem in a well-controlled experimental model. Accordingly, in this study, we assessed the effects of long-term maternal alcohol intake on offspring viability and growth as well as the DNA, total RNA, protein levels and DNA synthesis in brain, heart, liver, and kidney of 3-day-old newborn rats. Chronic oral intake of alcohol (mean 21 weeks plus 20 days of gestation) resulted in maternal blood alcohol levels of 67 to over 150 mg percent. There was a significant increase of newborn mortality (30%) and a decrease in their body weight at 3 days in alcohol-exposed compared to pair-fed (non-alcohol) control pups (p less than 0.025). Protein concentration was unchanged in heart, liver, and kidney and was slightly elevated in brain of 3-day-old pups exposed to ethanol in utero. DNA synthesis rates were normal in all four organs, while DNA concentration was significantly lower only in the liver of the alcohol-exposed group (p less than 0.05). In the alcohol group the total RNA levels were significantly depressed by about 10-30 percent (p less than 0.05) in all four organs studied. In conclusion, our studies suggest that prolonged maternal alcohol intake has an adverse effect on newborn rat viability and growth as well as the total RNA and to some extent DNA concentration of vital organs.
临床观察发现，酗酒孕妇的后代容易出现先天性缺陷以及生长发育异常（胎儿酒精综合征），这引发了人们在一个严格控制的实验模型中研究该问题的兴趣。因此，在本研究中，我们评估了长期母体摄入酒精对3日龄新生大鼠后代的活力和生长的影响，以及对其脑、心、肝、肾中的DNA、总RNA、蛋白质水平和DNA合成的影响。长期口服酒精（平均21周加20天妊娠期）导致母体血液酒精水平达到67至超过150毫克百分比。与配对喂养（非酒精）对照幼崽相比，暴露于酒精环境中的幼崽在3日龄时新生儿死亡率显著增加（30%），体重下降（p小于0.025）。暴露于子宫内乙醇的3日龄幼崽，其心脏、肝脏和肾脏中的蛋白质浓度没有变化，而脑中的蛋白质浓度略有升高。所有四个器官中的DNA合成率均正常，而DNA浓度仅在酒精暴露组的肝脏中显著降低（p小于0.05）。在酒精组中，所有四个研究器官中的总RNA水平均显著降低约10% - 30%（p小于0.05）。总之，我们的研究表明，母体长期摄入酒精对新生大鼠的活力和生长以及重要器官的总RNA和在一定程度上对DNA浓度有不利影响。
