Böni J, Emmerich B S, Leib S L, Wiestler O D, Schüpbach J, Kleihues P
Department of Pathology, University Hospital, Zürich, Switzerland.
Neurology. 1993 Sep;43(9):1813-7. doi: 10.1212/wnl.43.9.1813.
We analyzed brain tissue from 39 patients for the presence of proviral HIV-1 sequences, using the polymerase chain reaction (PCR) for the amplification of segments of the viral LTR and gag genes. A novel primer extension procedure allowed the detection of a single HIV-1 copy in 1 micrograms DNA. We detected proviral HIV-1 DNA in 16 of 25 brain samples from AIDS patients. Semiquantitative evaluation of the amplified DNAs indicated considerable variation in viral load. Highest levels of proviral DNA were present in brain samples from six patients with clinical evidence of HIV-associated cognitive/motor complex and the histopathologic correlate of HIV leukoencephalopathy or HIV encephalitis. An additional 11 brain samples contained smaller amounts of proviral DNA. In these patients, clinical data were inconclusive regarding the diagnosis of HIV-1 encephalopathy and histopathologically there was no evidence of HIV-1-induced tissue lesions. In nine of 25 seropositive patients with AIDS (36%), brain samples scored negative or did not contain an unequivocal signal indicating the presence of proviral DNA. HIV-1 sequences were not detected in any of 14 control brain samples from HIV-1 seronegative patients. Our data indicate that HIV-1 is present in the central nervous system of the majority (two thirds) of AIDS patients and that the highest levels of proviral DNA in brain tissue are associated with HIV encephalopathy.
我们使用聚合酶链反应（PCR）扩增病毒长末端重复序列（LTR）和gag基因片段，分析了39例患者脑组织中是否存在前病毒HIV-1序列。一种新的引物延伸程序能够在1微克DNA中检测到单个HIV-1拷贝。我们在25例艾滋病患者的脑样本中，有16例检测到前病毒HIV-1 DNA。对扩增DNA的半定量评估表明病毒载量存在显著差异。在6例有HIV相关认知/运动复合体临床证据且伴有HIV白质脑病或HIV脑炎组织病理学相关表现的患者脑样本中，前病毒DNA水平最高。另外11例脑样本中含有少量前病毒DNA。在这些患者中，关于HIV-1脑病的诊断，临床数据尚无定论，组织病理学上也没有HIV-1诱导组织损伤的证据。在25例艾滋病血清阳性患者中，有9例（36%）脑样本检测结果为阴性或未出现明确表明存在前病毒DNA的信号。在14例HIV-1血清阴性患者的对照脑样本中，未检测到HIV-1序列。我们的数据表明，大多数（三分之二）艾滋病患者的中枢神经系统中存在HIV-1，且脑组织中前病毒DNA的最高水平与HIV脑病相关。
