α-胸腺素在正常和异常生长的人体组织中的表达
Expression of alpha-thymosins in human tissues in normal and abnormal growth.
作者信息
Tsitsiloni O E, Stiakakis J, Koutselinis A, Gogas J, Markopoulos C, Yialouris P, Bekris S, Panoussopoulos D, Kiortsis V, Voelter W
机构信息
Zoological Laboratory, Faculty of Science, University of Athens, Greece.
出版信息
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9504-7. doi: 10.1073/pnas.90.20.9504.
Radioimmunoassays specific for the N and C termini of human prothymosin alpha and the N terminus of human parathymosin alpha were employed for the measurement of the levels of alpha-thymosins in human thymus, spleen, and liver during normal growth and intestine and breast in malignant growth. A differential expression of the two alpha-thymosins was observed in thymus (prothymosin alpha-rich) and liver (parathymosin alpha-rich). A decline in the levels of both alpha-thymosins was found with age, with prothymosin alpha in thymus showing the sharpest change (15- to 30-fold). The levels of both alpha-thymosins were higher in malignant tissues as compared with healthy ones. In breast cancer, in particular, the mean increase for prothymosin alpha and parathymosin alpha was 17.9- and 11.5-fold, respectively. The major crossreactive material was characterized in all cases as intact prothymosin alpha and parathymosin alpha. These results suggest an in vivo relationship of the expression of alpha-thymosins with the human tissue cell proliferation activity.
采用针对人原胸腺素α的N端和C端以及人副胸腺素α的N端的放射免疫分析法，来测定正常生长过程中人体胸腺、脾脏和肝脏以及恶性生长过程中肠道和乳腺中α-胸腺素的水平。在胸腺（富含原胸腺素α）和肝脏（富含副胸腺素α）中观察到两种α-胸腺素的差异表达。随着年龄增长，两种α-胸腺素的水平均下降，胸腺中的原胸腺素α变化最为明显（15至30倍）。与健康组织相比，恶性组织中两种α-胸腺素的水平更高。特别是在乳腺癌中，原胸腺素α和副胸腺素α的平均增加倍数分别为17.9倍和11.5倍。在所有情况下，主要交叉反应物质均被鉴定为完整的原胸腺素α和副胸腺素α。这些结果表明α-胸腺素的表达与人体组织细胞增殖活性之间存在体内关系。
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