File S E, Zangrossi H, Andrews N
UMDS Division of Pharmacology, Guy's Hospital, London, UK.
Pharmacol Biochem Behav. 1993 Aug;45(4):931-4. doi: 10.1016/0091-3057(93)90142-g.
In hippocampal and cortical slices taken from rats moved in their home cages to a novel environment for 5 min, there were decreases in basal and K(+)-evoked [14C]GABA release and an increased [14C]GABA uptake compared with slices taken from rats remaining undisturbed in the animal house. The changes in 5-hydroxytryptamine (5-HT) release and uptake in response to a novel environment were markedly time dependent. In rats killed immediately after the 5-min exposure, there was decreased hippocampal [3H]5-HT uptake and higher hippocampal basal release, whereas in rats killed 30 min later there was increased [3H]5-HT uptake and lower basal release in both hippocampal and cortical slices. Rats exposed to cat odor in the novel environment showed increased release and decreased uptake of GABA in both brain areas compared with the group exposed to a neutral odor in the same novel environment, and these differences between the two odor groups were found both immediately and 30 min after the odor exposure. In contrast, only one measure of 5-HT function differed between the neutral and cat odor groups, with the latter showing increased cortical [3H]5-HT uptake 30 min after odor exposure.
将处于饲养笼中的大鼠转移至新环境中5分钟后，取其海马体和皮质切片，与置于动物饲养室中未受干扰的大鼠的切片相比，基础和钾离子诱发的[14C]GABA释放减少，[14C]GABA摄取增加。5-羟色胺（5-HT）释放和摄取对新环境的反应变化明显具有时间依赖性。在暴露于新环境5分钟后立即处死的大鼠中，海马体[3H]5-HT摄取减少，海马体基础释放增加，而在30分钟后处死的大鼠中，海马体和皮质切片中的[3H]5-HT摄取增加，基础释放减少。与在相同新环境中暴露于中性气味的组相比，在新环境中暴露于猫气味的大鼠在两个脑区均表现出GABA释放增加和摄取减少，并且在气味暴露后立即和30分钟均发现两个气味组之间存在这些差异。相比之下，中性气味组和猫气味组之间只有一项5-HT功能指标存在差异，后者在气味暴露30分钟后皮质[3H]5-HT摄取增加。
