File S E, Zangrossi H, Andrews N
Psychopharmacology Research Unit, Guy's Hospital, London, U.K.
Neuropharmacology. 1993 Mar;32(3):217-21. doi: 10.1016/0028-3908(93)90103-a.
Changes in release and uptake of [3H]5-HT and [14C]GABA were compared in slices taken from the hippocampus and frontal cortex of rats, left undisturbed in their home-cages, or exposed for 5 min to the elevated plus-maze or social interaction tests of anxiety. Exposure to the plus-maze decreased cortical GABA function (shown by decreased release) and increased hippocampal 5-HT function (shown by increased K(+)-evoked release but more markedly by decreased uptake). Compared with undisturbed home cage controls, only the high light, familiar condition of the social interaction test resulted in a significant increase in K(+)-evoked release of both [3H]5-HT and [14C]GABA from the hippocampus. All four social interaction test conditions resulted in increases in cortical uptake of [3H]5-HT and all but the high light, unfamiliar condition increased cortical uptake of [14C]GABA. Analysing the two factors manipulated in the social interaction test, unfamiliarity with the test arena resulted in increased uptake of hippocampal [3H]5-HT and decreased cortical [14C]GABA, whereas an increase in the level of light decreased the cortical uptake of [14C]GABA. The results show that changes in presynaptic function occur rapidly in response to a brief exposure to animal tests of anxiety. However, only the increased hippocampal release of 5-HT is likely to be causally linked to anxiety and the results show that this cannot be the sole explanation.
比较了从大鼠海马体和额叶皮质切片中[3H]5-羟色胺（5-HT）和[14C]γ-氨基丁酸（GABA）的释放和摄取变化。这些大鼠被留在其饲养笼中不受干扰，或者暴露于高架十字迷宫或社交互动焦虑测试中5分钟。暴露于十字迷宫会降低皮质GABA功能（表现为释放减少），并增加海马体5-HT功能（表现为K⁺诱发的释放增加，但更明显的是摄取减少）。与不受干扰的饲养笼对照相比，只有社交互动测试中的强光、熟悉条件导致海马体中[3H]5-HT和[14C]GABA的K⁺诱发释放显著增加。所有四种社交互动测试条件均导致皮质对[3H]5-HT的摄取增加，除了强光、不熟悉条件外，其他条件均增加了皮质对[14C]GABA的摄取。分析社交互动测试中操纵的两个因素，对测试环境的不熟悉导致海马体[3H]5-HT摄取增加，皮质[14C]GABA摄取减少，而光照水平的增加则降低了皮质对[14C]GABA的摄取。结果表明，突触前功能的变化在短暂暴露于动物焦虑测试后迅速发生。然而，只有海马体中5-HT释放的增加可能与焦虑存在因果关系，并且结果表明这不能是唯一的解释。
