Powers D C
Geriatric Research, Education and Clinical Center, St. Louis VAMC, Missouri.
J Am Geriatr Soc. 1993 Jan;41(1):1-5. doi: 10.1111/j.1532-5415.1993.tb05938.x.
To investigate whether influenza A-specific cytotoxic T lymphocyte (CTL) activity is reduced in elderly compared with younger adults.
Case series comparing outcomes in young and elderly cohorts.
Saint Louis University Division of Geriatric Medicine.
Healthy adult outpatients and staff members aged < 50 (young) or > or = 65 (elderly) years.
Peripheral blood mononuclear cells were assayed for CTL activity by a 51chromium release assay following 1 week of in vitro stimulation with influenza A/PR/8/34 (H1N1) virus.
Percent specific lysis of autologous and allogeneic influenza virus-infected target cells.
Specific lysis of autologous A/PR-infected targets was significantly lower in elderly compared to young subjects (P < 0.01), and exceeded 10% in a significantly lower proportion of elderly compared with younger subjects (P < 0.05), but was not influenced by a history of vaccination within the preceding 12 months. Cytotoxic effectors were class I human leukocyte antigen (HLA)-restricted and displayed heterosubtypic cross-reactivity but were unable to lyse influenza B-infected targets.
These results demonstrate an age-related decline of influenza A virus-specific CTL activity and suggest that CTL responses to inactivated virus vaccine are of short duration.
研究与年轻成年人相比，老年人甲型流感特异性细胞毒性T淋巴细胞（CTL）活性是否降低。
比较年轻和老年队列结果的病例系列研究。
圣路易斯大学老年医学科。
年龄小于50岁（年轻组）或大于或等于65岁（老年组）的健康成年门诊患者和工作人员。
用甲型流感病毒A/PR/8/34（H1N1）体外刺激外周血单个核细胞1周后，采用51铬释放试验检测CTL活性。
自体和异体流感病毒感染靶细胞的特异性裂解百分比。
与年轻受试者相比，老年受试者中自体A/PR感染靶细胞的特异性裂解显著降低（P<0.01），且老年受试者中特异性裂解超过10%的比例显著低于年轻受试者（P<0.05），但不受前12个月内疫苗接种史的影响。细胞毒性效应细胞受I类人类白细胞抗原（HLA）限制，具有异亚型交叉反应性，但不能裂解乙型流感病毒感染的靶细胞。
这些结果表明甲型流感病毒特异性CTL活性存在与年龄相关的下降，并提示CTL对灭活病毒疫苗的反应持续时间较短。
