Schönfeld J V, Müller M K, Augustin M, Rünzi M, Goebell H
Department of Gastroenterology, University of Essen, Germany.
Dig Dis Sci. 1993 Jan;38(1):28-32. doi: 10.1007/BF01296769.
Cysteamine is known to deplete somatostatin from pancreatic D cells. In the isolated perfused rat pancreas we investigated its effects on somatostatin and insulin release as well as exocrine pancreatic secretion in the presence of 16.7 mM glucose and 180 pM CCK-8. At a concentration of 0.1 mM, cysteamine had no significant effect on pancreatic endocrine and exocrine functions. At 10 mM, however, cysteamine released somatostatin (380 +/- 70 vs 100 +/- 20 fmol/20 min), inhibited insulin output (890 +/- 120 vs 13210 +/- 3260 mu units/20 min) and reduced exocrine pancreatic secretion (volume: 12 +/- 2 vs 20 +/- 2 microliters/20 min; lipase: 31 +/- 3 vs 60 +/- 7 units/20 min). We conclude that the complex changes induced by cysteamine are consistent with a physiological role of endogenous somatostatin in the regulation of insulin release. The reduction of exocrine pancreatic secretion, however, was at least in part, if not completely, mediated via the insuloacinar axis rather than a direct effect of cysteamine-released somatostatin on pancreatic acinar cells.
已知半胱胺可使胰腺D细胞中的生长抑素耗竭。在离体灌注的大鼠胰腺中，我们研究了在16.7 mM葡萄糖和180 pM胆囊收缩素-8（CCK-8）存在的情况下，半胱胺对生长抑素和胰岛素释放以及胰腺外分泌的影响。在浓度为0.1 mM时，半胱胺对胰腺内分泌和外分泌功能无显著影响。然而，在10 mM时，半胱胺释放出生长抑素（380±70对100±20 fmol/20分钟），抑制胰岛素分泌（890±120对13210±3260微单位/20分钟），并减少胰腺外分泌（体积：12±2对20±2微升/20分钟；脂肪酶：31±3对60±7单位/20分钟）。我们得出结论，半胱胺诱导的复杂变化与内源性生长抑素在调节胰岛素释放中的生理作用一致。然而，胰腺外分泌的减少至少部分（如果不是完全）是通过胰岛-腺泡轴介导的，而不是半胱胺释放的生长抑素对胰腺腺泡细胞的直接作用。
