Yeatman T J, Updyke T V, Kaetzel M A, Dedman J R, Nicolson G L
Department of General Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Clin Exp Metastasis. 1993 Jan;11(1):37-44. doi: 10.1007/BF00880064.
Annexins are a large group of calcium-dependent cytoskeletal- and membrane-associated proteins whose properties include cytoskeleton and phospholipid binding and mitotic signal transduction. Although annexin-like molecules have been reported on the external plasma membranes of certain cells, in general they are considered to be cytoplasmic proteins. We report here the heterogenous expression of certain annexins (I-VI) on the external cell surfaces of non-metastatic and metastatic murine (RAW117 large-cell lymphoma), rat (13762NF mammary adenocarcinoma) and some human (KM12 and HT29 colorectal carcinoma) cell lines but not on some other cell lines such as human (A375 and MeWo) and mouse (B16) melanoma. The implication of annexin cell surface expression in the metastatic process is discussed with respect to tumor cell adhesion.
膜联蛋白是一大类依赖钙的细胞骨架和膜相关蛋白，其特性包括细胞骨架和磷脂结合以及有丝分裂信号转导。尽管在某些细胞的外部质膜上已报道有类似膜联蛋白的分子，但一般认为它们是细胞质蛋白。我们在此报告某些膜联蛋白（I - VI）在非转移性和转移性小鼠（RAW117大细胞淋巴瘤）、大鼠（13762NF乳腺腺癌）以及一些人类（KM12和HT29结肠直肠癌）细胞系的外部细胞表面的异质性表达，而在其他一些细胞系如人类（A375和MeWo）和小鼠（B16）黑色素瘤细胞系上则未表达。本文就肿瘤细胞黏附方面讨论了膜联蛋白细胞表面表达在转移过程中的意义。
