Vogel K G, Ordög A, Pogány G, Oláh J
Department of Biology, University of New Mexico, Albuquerque 87131.
J Orthop Res. 1993 Jan;11(1):68-77. doi: 10.1002/jor.1100110109.
Proteoglycan content and tissue morphology were examined in tendons and ligaments from 24 cadavers, ranging in age at the time of death from 1.5 months to 83 years. The region of the human tibialis posterior tendon that passes under the medial malleolus was characterized by cells having a rounded shape, positive staining with alcian blue, and higher glycosaminoglycanuronic acid content than in the more proximal region of the same tendon. Analysis of proteoglycans by sodium dodecyl sulfate/polyacrylamide gel electrophoresis indicated that the predominant small proteoglycan of the proximal/tensional region was decorin, whereas two types of small proteoglycans (decorin and biglycan) and large proteoglycans were present in the region passing under the medial malleolus and presumably subjected to compressive and shear forces in addition to tension. The pattern of proteoglycan accumulation in the compressed region of tendon was basically similar for all individuals and showed no distinctive trends related to age after puberty. In terms of type and amount of proteoglycan, the patellar tendon was like the tensional region of the tibialis posterior. Glycosaminoglycan content in the lateral collateral ligament and anterior cruciate ligament, however, was twofold higher than in the tendons. The ligaments contained large as well as small proteoglycans, just as in the compressed region of tendon.
对24具尸体的肌腱和韧带进行了蛋白聚糖含量及组织形态学检查，这些尸体死亡时的年龄从1.5个月到83岁不等。人类胫后肌腱在内踝下方通过的区域，其细胞呈圆形，阿尔辛蓝染色呈阳性，糖胺聚糖醛酸含量高于同一肌腱更近端的区域。通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳对蛋白聚糖进行分析表明，近端/张力区域主要的小蛋白聚糖是核心蛋白聚糖，而在内踝下方通过的区域存在两种小蛋白聚糖（核心蛋白聚糖和双糖链蛋白聚糖）以及大蛋白聚糖，该区域除了承受张力外，可能还受到压缩力和剪切力。所有个体肌腱受压区域的蛋白聚糖积累模式基本相似，青春期后未显示出与年龄相关的明显趋势。就蛋白聚糖的类型和数量而言，髌腱与胫后肌腱的张力区域相似。然而，外侧副韧带和前交叉韧带中的糖胺聚糖含量比肌腱中的高两倍。韧带中既有大蛋白聚糖也有小蛋白聚糖，就像肌腱的受压区域一样。
