Andreux J P, Merino A, Renard M, Forestier F, Cardot P
Laboratoire d'Hématologie, Chatenay Malabry, France.
Exp Hematol. 1993 Feb;21(2):326-30.
Field flow fractionation (FFF) is a new methodology described as being well-suited for the separation and characterization of biopolymers and particles. On theoretical grounds, cells may be separated with FFF if they differ in size, density or deformability. In the present study, we first tried to determine optimal separation conditions for red blood cells; thereafter we used FFF to examine red cell changes during a phenylhydrazine-induced hemolytic anemia. It has been shown that in less than 30 minutes, FFF is able to separate normal red blood cells from Heinz body-rich cells or reticulocytes that differ in size or density. The successive steps of hemolysis and regeneration appear clearly on the fractograms. Advantages and drawbacks of the method are discussed.
场流分级分离法（FFF）是一种新的方法，据称非常适合生物聚合物和颗粒的分离与表征。从理论上讲，如果细胞在大小、密度或可变形性方面存在差异，就可以用场流分级分离法进行分离。在本研究中，我们首先试图确定红细胞的最佳分离条件；此后，我们用场流分级分离法研究苯肼诱导的溶血性贫血过程中的红细胞变化。结果表明，在不到30分钟的时间内，场流分级分离法就能将正常红细胞与大小或密度不同的富含海因茨小体的细胞或网织红细胞分离。溶血和再生的连续步骤在分馏图上清晰可见。本文还讨论了该方法的优缺点。
