Tong X, Caldwell K D
Department of Bioengineering, University of Utah, Salt Lake City 84112, USA.
J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):39-47. doi: 10.1016/0378-4347(95)00297-0.
Human red blood cells were treated in different ways to alter their membrane deformability, and the hydrodynamic behavior of these altered cells was studied using the steric field-flow fractionation (FFF) technique. The relationships between cell retention in the FFF channel, flow-rate of the carrier fluid and the applied field strength were studied for normal and glutaraldehyde-fixed human red cells, and separation conditions were optimized. The effect of flow-induced hydrodynamic lift forces on red cell retention in the steric FFF channel was studied, and the results suggest that the membrane deformability of the red cell is an important factor contributing to the lift force, besides other previously described effects due to density and flow velocity. Using steric FFF, a mixture of normal and glutaraldehyde-fixed human red cells was completely separated with a resolution twice that found in published data from gel permeation, another hydrodynamic separation technique. Partial loss of membrane deformability, induced by different degrees of glutaraldehyde-fixation, by diamide, or by a thermal treatment, has also been studied. Steric FFF is thus shown to have potential for rapid separation and differentiation of red cells with different density and membrane deformability, conditions known to be associated with, e.g., cell senescence and certain hematological diseases.
人类红细胞经过不同处理以改变其膜变形性，并使用空间场流分级（FFF）技术研究这些改变后的细胞的流体动力学行为。针对正常和戊二醛固定的人类红细胞，研究了其在FFF通道中的保留情况、载液流速与外加场强之间的关系，并对分离条件进行了优化。研究了流动诱导的流体动力升力对红细胞在空间FFF通道中保留的影响，结果表明，除了先前描述的由于密度和流速产生的其他影响外，红细胞的膜变形性是导致升力的一个重要因素。使用空间FFF技术，正常和戊二醛固定的人类红细胞混合物被完全分离，分辨率是另一种流体动力学分离技术——凝胶渗透法已发表数据的两倍。还研究了由不同程度的戊二醛固定、二酰胺或热处理引起的膜变形性部分丧失。因此，空间FFF技术显示出具有快速分离和区分具有不同密度和膜变形性的红细胞的潜力，这些条件已知与例如细胞衰老和某些血液疾病有关。
