Kyzer M D, Aly A S, Davidson J M, Reinisch L, Ossoff R H
Department of Otolaryngology, Vanderbilt University Medical Center, Nashville, Tennessee.
Lasers Surg Med. 1993;13(1):62-71. doi: 10.1002/lsm.1900130112.
The KTP laser (wavelength 532 nm) was used in a sub ablative format to determine the effect of low energy density irradiation on the normal healing by primary intention of scalpel skin incisions in rats. Two longitudinal lased strips were created by a 1 cm diameter defocused beam on the shaved, cleaned dorsal epidermis of 32 Sprague-Dawley rates; one strip was produced with a 2.0 W beam (54 J, or 18 J/cm2 total dose), and the other with a 3.5 W beam (94.5 J or 31.5 J/cm2, total dose). Scalpel incisions were made longitudinally within the irradiated zones, using contra lateral scalpel incisions on unirradiated skin as controls. Tensiometric analysis of wound strength was performed at 3, 7, 14, and 23 days following surgery. The data from fresh tissue tensiometry indicate that KTP laser irradiation of skin incisions results in a lower tensile strength for the wound at 7 and 14 days. The decrease in tensile strength is proportional to the total energy density of the exposure. At day 3 and 23, the tensile strength of the wound was independent of the sub ablative laser exposure. The results are in general agreement with studies of the healing process of laser incisions and may help us to understand the details of the healing process from laser incisions.
采用KTP激光（波长532纳米）以亚消融模式来确定低能量密度照射对大鼠手术刀皮肤切口一期正常愈合的影响。在32只斯普拉格-道利大鼠剃毛并清洁后的背部表皮上，通过直径1厘米的散焦光束形成两条纵向激光照射条带；一条用2.0瓦光束产生（54焦耳，或总剂量18焦耳/平方厘米），另一条用3.5瓦光束产生（94.5焦耳或31.5焦耳/平方厘米，总剂量）。在照射区域内纵向进行手术刀切口，以未照射皮肤的对侧手术刀切口作为对照。在术后3天、7天、14天和23天对伤口强度进行张力分析。新鲜组织张力测量的数据表明，皮肤切口的KTP激光照射在第7天和第14天时导致伤口的抗张强度降低。抗张强度的降低与照射的总能量密度成正比。在第3天和第23天，伤口的抗张强度与亚消融激光照射无关。这些结果总体上与激光切口愈合过程的研究一致，可能有助于我们了解激光切口愈合过程的细节。
