Patriarca C, Verdier F, Brouland J P, Descotes J
Laboratoire d'Immunotoxicologie Fondamentale et Clinique, INSERM U80, Faculté de Médecine A. Carrel, Lyon, France.
Toxicol Lett. 1993 Jan;66(1):21-8. doi: 10.1016/0378-4274(93)90075-9.
The popliteal lymph node assay (PLNA) was proposed for the preclinical prediction of xenobiotics-induced autoimmune reactions in humans. Among the substances so far tested in this model, procainamide and isoniazid gave negative PLNA responses despite reports of lupus syndromes in man. To confirm the hypothesis that a metabolite instead of the parent molecule is involved, rats were pretreated with phenobarbital or beta-naphthoflavone, then injected with procainamide or isoniazid. In additional groups of animals, procainamide or isoniazid were injected together with S9-mix following various incubation times. Pretreated rats had a positive PLNA response when injected with procainamide, whereas preincubation with S9-mix resulted in a positive response to isoniazid. These results further support the validity of the PLNA.
腘淋巴结试验（PLNA）被用于临床前预测异生物素诱导的人类自身免疫反应。在该模型中迄今测试的物质中，尽管有人类狼疮综合征的报告，但普鲁卡因胺和异烟肼的PLNA反应为阴性。为了证实参与反应的是代谢物而非母体分子这一假设，用苯巴比妥或β-萘黄酮预处理大鼠，然后注射普鲁卡因胺或异烟肼。在另外几组动物中，在不同孵育时间后将普鲁卡因胺或异烟肼与S9混合物一起注射。用普鲁卡因胺注射预处理大鼠时PLNA反应呈阳性，而与S9混合物预孵育则导致对异烟肼的阳性反应。这些结果进一步支持了PLNA的有效性。
