Bardwell J C, Lee J O, Jander G, Martin N, Belin D, Beckwith J
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1038-42. doi: 10.1073/pnas.90.3.1038.
Protein disulfide bond formation in Escherichia coli requires the periplasmic protein DsbA. We describe here mutations in the gene for a second protein, DsbB, which is also necessary for disulfide bond formation. Evidence suggests that DsbB may act by reoxidizing DsbA, thereby regenerating its ability to donate its disulfide bond to target proteins. We propose that DsbB, an integral membrane protein, may be involved in transducing redox potential across the cytoplasmic membrane.
大肠杆菌中蛋白质二硫键的形成需要周质蛋白DsbA。我们在此描述了另一种蛋白质DsbB基因中的突变，DsbB对于二硫键的形成也是必需的。有证据表明，DsbB可能通过使DsbA重新氧化来发挥作用，从而恢复其将二硫键提供给靶蛋白的能力。我们提出，作为一种整合膜蛋白的DsbB可能参与跨细胞质膜传递氧化还原电位。
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