Maqueda M, Gálvez A, Martínez-Bueno M, Guerra I, Valdivia E
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
Antimicrob Agents Chemother. 1993 Jan;37(1):148-51. doi: 10.1128/AAC.37.1.148.
Antisera against the broad-spectrum peptide antibiotic AS-48 produced by Enterococcus faecalis were obtained from immunized rabbits. Appreciable antibody titers were obtained only after repeated immunization, suggesting a feeble antigenicity for AS-48. Upon incubation with AS-48, the antisera neutralized its bacteriolytic action on E. faecalis S-47, although the simultaneous addition of AS-48 and serum did not prevent lysis. Crude serum cross-reacted with outer envelope components of enterococci, although specific anti-AS-48 antibodies, purified by affinity chromatography, reacted only with AS-48-treated cells. Labelling with immunofluorescence and colloidal gold particles was carried out on sensitive and resistant bacterial species to determine the interaction of AS-48 with cell structures.
从免疫兔中获得了抗粪肠球菌产生的广谱肽抗生素AS-48的抗血清。仅在反复免疫后才获得可观的抗体滴度，这表明AS-48的抗原性较弱。与AS-48孵育后，抗血清中和了其对粪肠球菌S-47的溶菌作用，尽管同时添加AS-48和血清并不能阻止裂解。粗血清与肠球菌的外膜成分发生交叉反应，而通过亲和层析纯化的特异性抗AS-48抗体仅与经AS-48处理的细胞发生反应。对敏感和耐药细菌种类进行免疫荧光和胶体金颗粒标记，以确定AS-48与细胞结构的相互作用。
