Rarey K E, Curtis L M, ten Cate W J
Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville 32610.
Hear Res. 1993 Jan;64(2):205-10. doi: 10.1016/0378-5955(93)90007-n.
Individual, rat inner ear tissues were isolated and processed for determination of levels of glucocorticoid (GR) receptor by an Enzyme Linked Immuno-Sorbant Assay (ELISA). Differing levels of GR receptor between seven sampled inner ear regions were measured. Levels of GR receptors in the spiral ligament tissues were found to be significantly higher compared to all other tissue samples. GR levels in the tissues of stria vascularis and organ of Corti were different from one another but both were statistically higher than those detected in the vestibular tissue samples (dark cell regions, cristae ampullares and maculae utriculi), which had the lowest GR receptor levels measured. Intermediate levels of GR receptor were found in the endolymphatic sac region. It is suggested that the varying levels of inner ear GR receptors may be indicative of differing biological responses among the given tissues, as well as differences in the magnitudes of such responses to circulating glucocorticoids.
分离并处理个体大鼠的内耳组织，通过酶联免疫吸附测定（ELISA）来测定糖皮质激素（GR）受体水平。测量了七个采样内耳区域之间不同水平的GR受体。结果发现，螺旋韧带组织中的GR受体水平显著高于所有其他组织样本。血管纹和柯蒂氏器组织中的GR水平彼此不同，但两者在统计学上均高于在前庭组织样本（暗细胞区域、壶腹嵴和椭圆囊斑）中检测到的水平，前庭组织样本中的GR受体水平是所测样本中最低的。在内淋巴囊区域发现了中等水平的GR受体。研究表明，内耳GR受体水平的变化可能表明给定组织之间不同的生物学反应，以及这些组织对循环糖皮质激素反应程度的差异。
