Gen-Probe公司荚膜组织胞浆菌和新型隐球菌AccuProbes检测试剂的评估。
Evaluation of Gen-Probe's Histoplasma capsulatum and Cryptococcus neoformans AccuProbes.
作者信息
Huffnagle K E, Gander R M
机构信息
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235-9072.
出版信息
J Clin Microbiol. 1993 Feb;31(2):419-21. doi: 10.1128/jcm.31.2.419-421.1993.
Abstract
Gen-Probe's DNA probes were evaluated for use in the identification of clinical isolates of Histoplasma capsulatum var. capsulatum and Cryptococcus neoformans. Ninety-five mould-phase fungi were probed, including 41 isolates of H. capsulatum var. capsulatum. Similarly, 98 yeasts, including 42 C. neoformans isolates, were examined by using the C. neoformans DNA probe. In the study, both probes demonstrated 100% specificity and 100% sensitivity. Their use in the clinical laboratory may significantly reduce the time required for definitive identification of fungi.
摘要
对Gen-Probe公司的DNA探针进行了评估，以用于鉴定荚膜组织胞浆菌荚膜变种和新型隐球菌的临床分离株。检测了95株霉菌相真菌，其中包括41株荚膜组织胞浆菌荚膜变种分离株。同样，使用新型隐球菌DNA探针检测了98株酵母，其中包括42株新型隐球菌分离株。在该研究中，两种探针均显示出100%的特异性和100%的敏感性。它们在临床实验室中的应用可能会显著缩短明确鉴定真菌所需的时间。
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