抗真菌药物灰黄霉素对红细胞的体外光敏裂解作用。

In vitro photosensitized lysis of red blood cells by an antifungal drug griseofulvin.

作者信息

Fujita H, Inukai N, Matsuo I

机构信息

Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.

出版信息

J Photochem Photobiol B. 1993 Jan;17(1):77-80. doi: 10.1016/1011-1344(93)85010-6.

DOI:10.1016/1011-1344(93)85010-6

PMID:8433225

Abstract

Human red blood cells (RBCs) were lysed by in vitro irradiation in the presence of the antifungal drug griseofulvin (GF). Effects of UVA fluence and GF concentration on photohaemolysis were examined under aerobic conditions. The photohaemolysis occurred at much lower fluence than that necessary for oxidation of the membrane lipids. UVA-irradiated solution of GF did not cause haemolysis. The photohaemolysis was colloid osmotic in nature because it was preceded by K+ leakage from the cells and was delayed in the presence of 30 mM sucrose in the medium. Even under anaerobic conditions, RBCs were lysed by irradiation with higher fluence than that required for aerobic photohaemolysis. Therefore, some phototoxic mechanism other than photosensitized oxidation is also involved in the photohaemolysis.

摘要

在抗真菌药物灰黄霉素（GF）存在的情况下，通过体外照射使人类红细胞（RBCs）发生溶血。在有氧条件下，研究了UVA通量和GF浓度对光溶血的影响。光溶血发生时所需的通量远低于膜脂氧化所需的通量。GF的UVA照射溶液不会引起溶血。光溶血本质上是胶体渗透压性的，因为其发生之前细胞会发生钾离子泄漏，并且在培养基中存在30 mM蔗糖时会延迟。即使在厌氧条件下，红细胞通过比有氧光溶血所需通量更高的照射也会发生溶血。因此，除了光敏氧化之外，某些其他光毒性机制也参与了光溶血过程。