Erickson A L, Walker C M
Chiron Corporation, Emeryville, California 94608.
J Gen Virol. 1993 Apr;74 ( Pt 4):751-4. doi: 10.1099/0022-1317-74-4-751.
Cytotoxic T lymphocyte (CTL) responses to vaccinia virus (VV) were studied in human subjects receiving smallpox vaccine by dermal scarification. After in vitro restimulation with VV, peripheral blood mononuclear cells (PBMCs) from three of six vaccinated subjects killed virus-infected target cells. VV-specific, HLA-restricted CTL activity was mediated primarily by CD8+ cells, although low levels of lytic activity by CD4+ cells were observed in some experiments. Two of the three responders had no history of exposure to VV prior to vaccination, whereas all three non-responders were vaccinated against smallpox during childhood. PBMCs from an additional three subjects who had received smallpox vaccine at least 20 years previously were negative for CTL activity. These data suggest that the duration of memory CTL populations against VV in the peripheral blood is limited, and that pre-existing immunity to VV may interfere with boosting of the CTL response upon revaccination.
通过皮肤划痕接种天花疫苗的人体受试者中，研究了对痘苗病毒（VV）的细胞毒性T淋巴细胞（CTL）反应。用VV进行体外再刺激后，六名接种疫苗受试者中的三名的外周血单核细胞（PBMC）杀死了病毒感染的靶细胞。尽管在一些实验中观察到CD4 +细胞有低水平的裂解活性，但VV特异性、HLA限制的CTL活性主要由CD8 +细胞介导。三名有反应者中的两名在接种疫苗之前没有接触过VV的病史，而三名无反应者在儿童时期都接种过天花疫苗。另外三名至少在20年前接种过天花疫苗的受试者的PBMC的CTL活性为阴性。这些数据表明，外周血中针对VV的记忆CTL群体的持续时间是有限的，并且预先存在的对VV的免疫力可能会干扰再次接种时CTL反应的增强。
