脑源性成纤维细胞生长因子：与髓磷脂碱性蛋白的一个片段相同。
Brain-derived fibroblast growth factor: identity with a fragment of the basic protein of myelin.
作者信息
Westall F C, Lennon V A, Gospodarowicz D
出版信息
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4675-8. doi: 10.1073/pnas.75.10.4675.
Abstract
Fibroblast growth factors (FGF) isolated from bovine brain have been identified chemically and immunologically as components of the myelin basic protein. The intact bovine basic protein molecule (170 residues), prepared by the standard acid extraction procedure, lacked mitogenic activity (tested at concentrations up to 10 microgram/ml). However, the polypeptide FGF-2, identified as residues 44-153 of the basic protein, was maximally mitogenic for fibroblasts at 10 ng/ml and polypeptide 44-166 (FGF-1) was maximally active at 100 ng/ml. Pituitary-derived FGF is a potent a growth factor as FGF-2, but appears to be biochemically and immunologically distinct from brain-derived FGF. FGF released in the central or peripheral nervous system as a consequence of myelin damage and basic protein proteolysis could provide a physiological stimulus for wound healing and myelin repair.
摘要
从牛脑中分离出的成纤维细胞生长因子（FGF）已通过化学和免疫学方法鉴定为髓鞘碱性蛋白的组成成分。采用标准酸提取程序制备的完整牛碱性蛋白分子（170个残基）缺乏促有丝分裂活性（在浓度高达10微克/毫升时进行测试）。然而，被鉴定为碱性蛋白第44 - 153位残基的多肽FGF - 2在10纳克/毫升时对成纤维细胞具有最大促有丝分裂活性，而多肽44 - 166（FGF - 1）在100纳克/毫升时具有最大活性。垂体来源的FGF与FGF - 2一样是一种有效的生长因子，但在生化和免疫学上似乎与脑来源的FGF不同。由于髓鞘损伤和碱性蛋白蛋白水解而在中枢或外周神经系统中释放的FGF可为伤口愈合和髓鞘修复提供生理刺激。
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