Sidhu M S, Menonna J P, Cook S D, Dowling P C, Udem S A
Neurology Service, VA Medical Center, East Orange, New Jersey 07019.
Virology. 1993 Mar;193(1):50-65. doi: 10.1006/viro.1993.1102.
We cloned the genomic RNA of canine distemper virus (CDV) and determined the nucleotide sequence of the large (L) protein-coding gene. The L gene is 6573 nucleotides long and contains a single open reading frame coding for a polypeptide of 2161 amino acids (MW 246,354). The precise 5' end of the viral genome consists of a 38-nucleotide leader region. The CDV L protein shows over 77% amino acid similarity with its morbilliform relative measles virus (MV) with nearly 67% of their amino acids conserved. The sequence homology of 11 negative strand viruses L proteins is compared and relatedness was found in the following decreasing order: CDV, MV, Sendai virus, parainfluenza virus type 3, simian virus 5, parainfluenza virus type 2, mumps virus, Newcastle disease virus, respiratory syncytial virus, vesicular stomatitis virus, rabies virus. The consensus sequence of proposed functional domains involved in L gene catalytic activities was well conserved in the CDV L protein.
我们克隆了犬瘟热病毒（CDV）的基因组RNA，并确定了大（L）蛋白编码基因的核苷酸序列。L基因长6573个核苷酸，包含一个单一的开放阅读框，编码一个由2161个氨基酸组成的多肽（分子量246,354）。病毒基因组的精确5'端由一个38个核苷酸的前导区组成。CDV L蛋白与其麻疹样相关病毒麻疹病毒（MV）的氨基酸相似性超过77%，其氨基酸近67%保守。比较了11种负链病毒L蛋白的序列同源性，发现相关性按以下降序排列：CDV、MV、仙台病毒、3型副流感病毒、猿猴病毒5、2型副流感病毒、腮腺炎病毒、新城疫病毒、呼吸道合胞病毒、水疱性口炎病毒、狂犬病病毒。参与L基因催化活性的拟议功能域的共有序列在CDV L蛋白中保存良好。
