Springael D, Diels L, Hooyberghs L, Kreps S, Mergeay M
Laboratory of Genetics and Biotechnology, SCK/CEN-VITO, Mol, Belgium.
Appl Environ Microbiol. 1993 Jan;59(1):334-9. doi: 10.1128/aem.59.1.334-339.1993.
Alcaligenes eutrophus strains exhibiting both plasmid-borne heavy metal resistance and haloaromatic-degrading functions were obtained by intraspecific conjugation. The strains which we constructed expressed catabolic and resistance markers together. Degradation of various polychlorinated biphenyl isomers and 2,4-D (2,4-dichlorophenoxyacetic acid) was observed in the presence of 1 mM nickel or 2 mM zinc, provided that the metal resistance determinant was present in the catabolizing strain. Such strains may be useful for decontamination of sites that are polluted with both organic compounds and heavy metals.
通过种内接合获得了兼具质粒介导的重金属抗性和卤代芳烃降解功能的嗜中温产碱杆菌菌株。我们构建的菌株同时表达了分解代谢和抗性标记。在存在1 mM镍或2 mM锌的情况下，观察到各种多氯联苯异构体和2,4-D（2,4-二氯苯氧基乙酸）的降解，前提是分解代谢菌株中存在金属抗性决定因素。这类菌株可能有助于对同时受到有机化合物和重金属污染的场地进行去污处理。
