Traganos F, Darzynkiewicz Z, Sharpless T, Melamed M R
Int J Cancer. 1977 Jul 15;20(1):30-6. doi: 10.1002/ijc.2910200107.
Five human bladder cell lines, four derived from tumor tissue and the fifth originating from presumed benign transitional epithelium, were examined by flow cytofluorometry to estimate the DNA and RNA content per cell during exponential and stationary phases of growth. A new staining technique was employed using acridine orange to differentially stain DNA and RNA in unfixed cells made permeable to the dye and other reagents by treatment with detergent at low ph. Stemline chromosome numbers for each cell line correlated well with relative DNA content of the G1 population as measured by this technique. In addition, the simultaneous measurements of DNA and RNA per cell yielded cell cycle distributions for each cell line. The ratio of stainable RNA/DNA was lower for all cell lines derived from bladder tumors as compared to the presumed normal cell line, indicating high nuclear/cytoplasmic ratios for the former.
对五种人膀胱细胞系进行了流式细胞荧光测定，其中四种源自肿瘤组织，第五种源自假定的良性移行上皮，以估计细胞在指数生长期和静止期的DNA和RNA含量。采用一种新的染色技术，使用吖啶橙对未固定细胞中的DNA和RNA进行差异染色，通过在低pH值下用去污剂处理使细胞对染料和其他试剂具有通透性。通过该技术测量，每个细胞系的干系染色体数与G1期群体的相对DNA含量密切相关。此外，对每个细胞系中DNA和RNA的同时测量得出了细胞周期分布。与假定的正常细胞系相比，所有源自膀胱肿瘤的细胞系中可染色RNA/DNA的比例较低，表明前者的核/质比例较高。
