Iida K, Moriyama K, Matsumoto S, Kawasaki H, Nishida E, Yahara I
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
Gene. 1993 Feb 14;124(1):115-20. doi: 10.1016/0378-1119(93)90770-4.
We have cloned a Saccharomyces cerevisiae gene (COF1) encoding a low-M(r) actin-binding protein of 143 amino acid (aa) residues (yeast cofilin; Cof); its aa sequence is 35% identical to porcine Cof. The yeast recombinant Cof produced in Escherichia coli exhibited in vitro activities on actin filaments similar to those of mammalian and avian Cof. Gene disruption and tetrad analysis showed that gene COF1 is essential for yeast cell growth. Expression of the cDNA of porcine Cof or destrin (Des), the latter a Cof-related protein, complemented the cof1 null allele in yeast cells.
我们克隆了酿酒酵母基因（COF1），该基因编码一种由143个氨基酸残基组成的低分子量肌动蛋白结合蛋白（酵母丝切蛋白；Cof）；其氨基酸序列与猪丝切蛋白的一致性为35%。在大肠杆菌中产生的酵母重组Cof在体外对肌动蛋白丝表现出与哺乳动物和禽类Cof相似的活性。基因破坏和四分体分析表明，COF1基因对酵母细胞生长至关重要。猪丝切蛋白或肌动蛋白解聚因子（Des，后者是一种与丝切蛋白相关的蛋白）的cDNA表达可互补酵母细胞中的cof1无效等位基因。
