Wheeler C M, Garcia Monco J C, Benach J L, Golightly M G, Habicht G S, Steere A C
State of New York Department of Health, State University of New York, Stony Brook 11794.
J Infect Dis. 1993 Mar;167(3):665-74. doi: 10.1093/infdis/167.3.665.
Preparative thin-layer chromatograms of chloroform-methanol extracts of Borrelia burgdorferi (B31) sonicates showed four fractions (Rf values of 0.84, 0.81, 0.66 and 0.61) that stained with iodine vapors, orcinol, or phospray, suggesting the presence of lipid-, carbohydrate-, and phosphorus-containing compounds. Sera from patients with Lyme disease showed IgM or IgG antibody reactivity to hydrophobic fractions, designated F1 and F2, in both early and late stages of the disease. Lack of constitutive amino acids in these fractions was shown by protein, amino acid, and peptide detection analyses. Sera from patients with syphilis, systemic lupus erythematosus, and antiphospholipid syndrome reacted to one or both of the fractions. Adsorption of sera from Lyme disease patients with intact B. burgdorferi resulted in significantly different pre- and postadsorption patterns of reactivity by whole cell ELISA, whereas adsorption with F1 and F2 resulted in similar pre- and postadsorption patterns. These fractions may not be present in aqueous whole cell or whole cell lysate ELISA antigens or in immunoblots.
伯氏疏螺旋体（B31）超声裂解物的氯仿 - 甲醇提取物的制备型薄层色谱图显示有四个馏分（Rf值分别为0.84、0.81、0.66和0.61），这些馏分用碘蒸气、间苯二酚或磷钼酸染色，表明存在含脂质、碳水化合物和磷的化合物。莱姆病患者的血清在疾病的早期和晚期均显示出IgM或IgG抗体对疏水馏分（命名为F1和F2）的反应性。蛋白质、氨基酸和肽检测分析表明这些馏分中缺乏组成性氨基酸。梅毒、系统性红斑狼疮和抗磷脂综合征患者的血清对其中一个或两个馏分有反应。用完整的伯氏疏螺旋体吸附莱姆病患者的血清，通过全细胞ELISA检测，吸附前后的反应模式有显著差异，而用F1和F2吸附则导致吸附前后的反应模式相似。这些馏分可能不存在于水性全细胞或全细胞裂解物ELISA抗原或免疫印迹中。
